Troponin C (TnC)

• solved by MIR (Herzberg & James, 1985)
• data kindly supplied by Osnat Herzberg, with assistance from Marie Fraser

• data: original 2.8 Å native data set
  • 3868 reflections
  • 496 flagged as cross-validation data

• phase information: SIR - single () derivative
  • original heavy atom parameters further refined using MLPHARE
  • relatively poor phases
  • MLPHARE reported a mean FOM of 0.39
  • 0.29 mean cosine phase difference with final model

• starting model: generated by 'scrambling' the final model
  • 2.28 Å rmsd with final model
  • 0.21 mean cosine phase difference with final model